Basic Information About Evaporative Light Scattering Detectors

By Roger Carter


HLPC is that technique utilized on analytical chemistry in separating, quantifying and identifying each component within a mixture. This is being used in research, medical, manufacturing and legal purposes. Samples are in separating components of complex sample, detecting vitamin levels within blood serum, producing biological or pharmaceutical products and detecting drugs in the urine.

Chromatography might be described as a process in mass transfer involving adsorption. This relies on pumps in passing a pressurized liquid and sample mixture through the column filled with an adsorbent which results in the sample components being separated. HLPS could be used in conjunction with Evaporative Light Scattering Detectors to analyze compounds where ultraviolet detection may be restricted such as in alcohols, terpenoids, phospholipids, lipids, antibiotics, antivirals and sugars.

ELSDs are detectors of HLPC based on aerosol working through to covert eluents of these target components to spray with a nebulizer. This then is heated so that only the mobile phase evaporates and the remaining target substance is focused with light to detect the scattered ones. Its primary advantage to others is the ability of identifying those cannot be detected by UV detection methods.

ELSD has many applications as it is among the universal detectors HPLC has available and is a detector ideal for natural substance analysis, combinatorial chemistry and drug discovery. Inert, dry and clean gas supply is required by this to carry the eluents. Example of this is Nitrogen gas which must be pure to remove the safety concerns, hassle and inconvenience inherent to others.

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