TCO or Trans cyclooctene has been innovated and studied along with another component which is tetrazine. The reaction between the conjugations of this two became the TCO Click Chemistry which is typically preceding without the use of copper. In addition, it has no means of connection with the rest of catalyst type. This mix has had a rapid and fully biorthogonal which excels on a low concentration unlike other reactions to be ever applied on the history of such matters. Due to that, it became a method for biorthogonal labeling as well as crosslinking choice.
However, for its application biologically speaking, there has to be a classical copper promoted one, three of dipolar cycloaddition which were developed by Meldal and Sharpless. However, it is not an ideal choice. This is because of the cytotoxity which were readily spotted and found on coppers. Without it, it promotes an alkyne azide cycloaddition instead.
However, the very first study which was created was said to have involved a copper material along. It was the idea of Meldal and Sharpless to begin with which was not that successful enough since it was not that ideal in the end. There are cytotoxity found on copper that made it less recommended in such procedures.
Thus, not having it on the mixture could promote alkyne azide cycloaddition on the other hand. This mix that does not involve copper was a product of the brilliant mind of Bertozzi. The only drawback with this study is that utilization of the cyclooctynes was made limited because its reaction were usually moderated to the kinetics. Because of that, its proven to be unsuitable for application to some biological system that has lower biomolecule creation.
Thus making the entire mix not suitable for biological system application though not all, still its biomolecule creation is relatively lower. This moderation has affected the compound in ways. It backfired to several effects.
This is the reason why the properties of tetrzine in the click chemistry has the method of choice for the labeling and crosslinking of its biomolecules in the living cells. Tetrazines has apparently two main types and is widely applied. Six methyl substituted tetrazine is one and the other would be the six hydrogen substituted tetrazines.
The one with methyl substitute has exhibit a relatively high stability even if its dissolve to aqueous media. But, it still is offering a fast reaction into the kinetics with the TCO derivatives than any of the biorthogonal reaction pairs. Thus, they as well are known to tolerate a vast array of reaction conditions.
This kind of tetrazine makes a prime choice for any application such as protein labeling. On the other hand, one with the hydrogen substitute has shown a lower stability and an obvious less tolerance to any harsh reaction condition. However, it offers an extremely fast reactions to the kinetics. This reaction could be seen in the application like vivo imaging.
The other type is six hydrogen substituted which apparently is an opposite of the latter. It only has a low stability along with its less tolerance to reaction that are harsh enough. But, this is not plain useless even if its weak because it can still react in rapid form to kinetics. Because of that property, it became preferable in some vivo imaging application.
However, for its application biologically speaking, there has to be a classical copper promoted one, three of dipolar cycloaddition which were developed by Meldal and Sharpless. However, it is not an ideal choice. This is because of the cytotoxity which were readily spotted and found on coppers. Without it, it promotes an alkyne azide cycloaddition instead.
However, the very first study which was created was said to have involved a copper material along. It was the idea of Meldal and Sharpless to begin with which was not that successful enough since it was not that ideal in the end. There are cytotoxity found on copper that made it less recommended in such procedures.
Thus, not having it on the mixture could promote alkyne azide cycloaddition on the other hand. This mix that does not involve copper was a product of the brilliant mind of Bertozzi. The only drawback with this study is that utilization of the cyclooctynes was made limited because its reaction were usually moderated to the kinetics. Because of that, its proven to be unsuitable for application to some biological system that has lower biomolecule creation.
Thus making the entire mix not suitable for biological system application though not all, still its biomolecule creation is relatively lower. This moderation has affected the compound in ways. It backfired to several effects.
This is the reason why the properties of tetrzine in the click chemistry has the method of choice for the labeling and crosslinking of its biomolecules in the living cells. Tetrazines has apparently two main types and is widely applied. Six methyl substituted tetrazine is one and the other would be the six hydrogen substituted tetrazines.
The one with methyl substitute has exhibit a relatively high stability even if its dissolve to aqueous media. But, it still is offering a fast reaction into the kinetics with the TCO derivatives than any of the biorthogonal reaction pairs. Thus, they as well are known to tolerate a vast array of reaction conditions.
This kind of tetrazine makes a prime choice for any application such as protein labeling. On the other hand, one with the hydrogen substitute has shown a lower stability and an obvious less tolerance to any harsh reaction condition. However, it offers an extremely fast reactions to the kinetics. This reaction could be seen in the application like vivo imaging.
The other type is six hydrogen substituted which apparently is an opposite of the latter. It only has a low stability along with its less tolerance to reaction that are harsh enough. But, this is not plain useless even if its weak because it can still react in rapid form to kinetics. Because of that property, it became preferable in some vivo imaging application.
About the Author:
If you wish to become informed about the TCO click chemistry you should first review the information online. We have revealed all the facts on http://www.conju-probe.com/product-category/tetrazine-reagents-tco-reagents/tco-reagents.